Download Applied Virology Research: New Diagnostic Procedures by Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), PDF

By Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), Edouard Kurstak, R. G. Marusyk, F. A. Murphy, M. H. V. Van Regenmortel (eds.)

Volume three is dedicated to the most recent diagnostic know-how for virus illnesses, relatively molecular methodologies.

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1989). Based on this type of analysis it can be estimated that approximately 10% of the infected cells in asymptomatic persons are actively expressing virus, compared to virtually 100% of the infected cells in symptomatic persons. These results indicate that as patients progress from an asymptomatic to a symptomatic state the number of infected cells and the proportion of infected cells actively expressing HIV increases substantially. This is consistent with a direct and probable causal relation between increase in viral burden, and immunosuppression and disease, presumably do to the increase in HIV expression leading to eventual destruction of CD4+ cells.

1989). PCR has advantages that highlight its usefulness as an early and rapid diagnostic test for newborns. These include: a) its ability to detect HIV proviral DNA and therefore avoid the problem of maternal antibody; b) it can be performed on 2-3 ml of blood, a volume that can practically be obtained from a newborn; c) it is rapid and can be completed in one days time; and d) it is less biohazardous than HIV culture. , 1990). , 1990). Most infants were found to be PCR positive by 6 months of age.

Mullis and developed by co-workers (Mullis, 1990). , 1988). Each cycle of replication is preceded by periods of thermal denaturation (Figure 1A). Typically, greater than twenty cycles of PCR are required to produce better than 1ot>-fold amplification of a target sequence. , 1988; Erlich, 1989; Persing and Landry, 1989). , 1990). Importantly, PCR amplification has found extensive application for detecting human immunodeficiency virus (HIV-1). , 1991). Since the first report of the PCR in vitro target amplification system, other nucleic acid target amplification methods have been described, exploiting the properties of different enzymatic reactions.

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